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KMID : 0362220130400040263
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Journal of The Korean Research Society for Dental Materials
2013 Volume.40 No. 4 p.263 ~ p.269
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Evaluation of cytotoxicity of 10 dentin desensitizers
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Son Kyung-Mi
Kwon Ji-Hyung
Park Hee-Chul
Zhu Ting Ting
Lim Bum-Soon
Yang Hyeong-Cheol
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Abstract
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Dentin desensitizer is being widely used to treat hypersensitivity of teeth in dental clinics, and a variety of dentin desensitizers are commercially available. Because dentin desensitizers are applied close to oral tissues including gingiva and pulp, it is necessary to evaluate cytotoxicity of 10 desensitizers to secure the biological safety of the materials. In this study, we investigated cytotoxicity of dentin desensitizers by two different evaluation methods, mitochondrial dehydrogenase activity test with WST-8 and agar diffusion assay with neutral red. In the test with WST-8, Gluma Desensitizer, MicroPrime G, HurriSeal and Cervial Cement showed high cytotoxicity, followed by Zarosen, UltraEZ and Systemp desensitizer. In agar diffusion assay, Gluma Desensitizer, MicroPrime G and HurriSeal exhibited severe cytotoxicity, while other desensitizers showed moderate or mild cytotoxicity. Cervical Cement which exhibited relatively high toxicity in WST-8 assay showed mild cytotoxicity in the agar diffusion assay, of which difference was likely due to the difference of evaluation mechanism between WST-8 and neutral red. The highly cytotoxic Gluma Desensitizer, MicroPrime G, HurriSeal contain both or either of glutaraldehyde and 2-hydroxylethyl methacrylate (HEMA), suggesting that glutaraldehyde and HEMA induced strong cytotoxicity of the desensitizers.
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KEYWORD
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Dentin desensitizer, cytotoxicity, mitochondrial dehydrogenase activity, WST-8, agar diffusion assay, neutral red
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